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DOI Prefix 10.20431 |
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Enhancement of Hydrogen Production by Combining a Blend Ratio for Two Types of Algal Waste Biomass and Reducing Casamino Acids Supplementation By Clostridium butyricum RAK25832
Shahira Said Aly1,3,Tsuyoshi Imai2*,Mohamed Salah Hassouna3,Takaya Higuchi2,Ariyo Kanno2,Koichi Yamamoto2,Rinji Akada2,Masahiko Sekine2
2.Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi, Japan.
3.Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University,Alexandria, Egypt.
Citation :Tsuyoshi Imai,et.al, Enhancement of Hydrogen Production by Combining a Blend Ratio for Two Types of Algal Waste Biomass and Reducing Casamino Acids Supplementation By Clostridium butyricum RAK25832 International Journal of Research in Environmental Science 2018,4(1) : 9-19
A previous study by the present authors identified nutrients necessary for hydrogen production, which was enhanced by using casamino acids (CAAs) as a nitrogen source. However, CAAs are costly; accordingly, using algal waste substrates with different nutrient compositions was speculated to serve as an alternative strategy for increasing hydrogen production from biomass. The present findings showed that optimizing the levels of the minerals FeCl2, NaHCO3, MgCl2, and K2HPO4 improved hydrogen yield (HY) to 107 ml H2/volatile solids (VS). Using Chlorella fusca or Ulva lactuca as a single substrate, the cultures required CAAs supplementation at concentrations of 13.33 and 6.67 g/l, respectively. However, using a blend of both substrates at a ratio 4 : 1 reduced the CAAs requirement to 3.33 g/l, with a HY of 117 ml H2/VS. Thus, feedstocks consisting of biomass from different sources reduce the amount of CAAs required, therefore decreasing cost whilst increasing effective energy production