The present study provides the first scientific information on the length-weight relationship for Macrobrachium sp. from Barito River, Indonesia. A total of 336 specimens consisted of 161 males (48%) and 175 females (52%) with the sex ratio of 0.92:1 were analysed. This species were collected by using light traps with various intensities and trap designs. The statistical analysis confirmed that males showed positive allometric growth (b=3.210), while females displayed negative allometric growth (b=2.734).Males had total length (TL), carapace length (CL) and chelae length (ChL) longer than females. The CL/TL, ChL/TL and CL/ChL of males were considerably higher than that of females (P< 0.001). Males were also significantly heavier than females. The estimated mean weight ranged from 0.454 to 10.015 g corresponding to the mean total length ranged from 27.38 to 81.64 mm. The condition factor values ranged from 1.00 to 2.19, indicating shrimp living in Barito River are in better condition. From fishing point of view, Macrobrachium sp. showed positively responded to all light traps tested. For future application, the use of LED light trap is strongly recommended.